Coding

Part:BBa_K1039029:Design

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-12)


BXB1 integrase with Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
    Illegal XhoI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1253
    Illegal AgeI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


Design Notes

A scar was added between the integrase and BBa_K608002


Source

The DNA was synthesized through New England BioLabs

References